Back to Search
Start Over
RAST-detection of specific IgE in diphenylmethane diisocyanate exposed workers: considerations in performance of the test.
- Source :
- Allergy; Apr91, Vol. 46 Issue 3, p166-172, 7p
- Publication Year :
- 1991
-
Abstract
- Some parameters which might affect RAST results, i.e. incubation length, degree of ligand substitution, and total IgE levels, were examined in a RAST system employing diphenylmethane diisocyanate--human serum albumin (MDI-HSA) conjugates as antigens, both in exposed symptomatic and in non-exposed subjects. The reaction equilibrium was reached after 9 and 18 h. respectively, in the first and the second step of the test. A marked correlation with the degree of ligand substitution was observed, the highest sensitivity being achieved with the lowest (2.9) MDI/HSA molar ratio examined. Higher (up to 50) degrees of substitution resulted in progressively lower levels of radioactivity bound and in a loss of specificity, as confirmed by RAST inhibition experiments. At high levels of ligand substitution, high titers of total serum IgE affected RAST results. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01054538
- Volume :
- 46
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 14203370
- Full Text :
- https://doi.org/10.1111/j.1398-9995.1991.tb00565.x