Back to Search
Start Over
Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor.
- Source :
- Chinese Journal of Integrative Medicine; Apr2020, Vol. 26 Issue 4, p270-276, 7p
- Publication Year :
- 2020
-
Abstract
- Objective: To study the effects of Prunella vulgaris polysaccharide (PVP) on human breast carcinoma-associated fibroblasts (CAFs). Methods: Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)-2H-tetrazolium (MTS) assay. Wound healing experiment and transwell migration assay were used to investigate the anti-migration effects. Flow cytometry was applied to detect cell apoptosis and cell cycle distribution. Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor (bFGF) in CAFs. Culture SKBr-3 with CAFs conditioned medium (CAFs-CM) to evaluate the indirect function on the proliferation of breast cancer SKBr-3 cells. Results: PVP inhibited the viability of CAFs by inducing apoptosis (P <0.01) and arresting cell cycle (P <0.01). It also inhibited the migration of CAFs (P <0.01). bFGF promoted CAFs proliferation (P <0.01) and migration (P <0.01), protected CAFs from apoptosis (P <0.05) and reduced G<subscript>0</subscript> phase to 49.06% (P <0.01). However, these effects of bFGF on CAFs could be abrogated by PVP. Culturing SKBr-3 with CAFs-CM, PVP could inhibit the viability of breast cancer SKBr-3 cells indirectly. Moreover, PVP reduced the mRNA expression (P <0.01) and protein secretion of bFGF (P <0.01) in CAFs. Conclusion: PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expression, thus inhibiting the growth of breast cancer SKBr-3 cells indirectly. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL proliferation
APOPTOSIS
BREAST tumors
CANCER
CELL physiology
CELL motility
CHALONES
CHEMICAL reagents
ENZYME-linked immunosorbent assay
FIBROBLASTS
FLOW cytometry
GENE expression
GROWTH factors
CHINESE medicine
POLYMERASE chain reaction
POLYSACCHARIDES
PLANT extracts
PHENOMENOLOGICAL biology
REVERSE transcriptase polymerase chain reaction
CELL survival
DESCRIPTIVE statistics
Subjects
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 26
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 142471279
- Full Text :
- https://doi.org/10.1007/s11655-016-2587-x