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Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance.

Authors :
Yu, Bolun
Jiang, Huifang
Pandey, Manish K.
Huang, Li
Huai, Dongxin
Zhou, Xiaojing
Kang, Yanping
Varshney, Rajeev K.
Sudini, Hari K.
Ren, Xiaoping
Luo, Huaiyong
Liu, Nian
Chen, Weigang
Guo, Jianbin
Li, Weitao
Ding, Yingbin
Jiang, Yifei
Lei, Yong
Liao, Boshou
Source :
Toxins; Mar2020, Vol. 12 Issue 3, p156, 1p
Publication Year :
2020

Abstract

Aflatoxin B<subscript>1</subscript> (AFB<subscript>1</subscript>) and aflatoxin B<subscript>2</subscript> (AFB<subscript>2</subscript>) are the most common aflatoxins produced by Aspergillus flavus in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA markers associated with resistance to aflatoxin production is likely to offer breeders efficient tools to develop resistant cultivars through molecular breeding. In this study, seeds of 99 accessions of a Chinese peanut mini-mini core collection were investigated for their reaction to aflatoxin production by a laboratory kernel inoculation assay. Two resistant accessions (Zh.h0551 and Zh.h2150) were identified, with their aflatoxin content being 8.11%–18.90% of the susceptible control. The 99 peanut accessions were also genotyped by restriction site-associated DNA sequencing (RAD-Seq) for a genome-wide association study (GWAS). A total of 60 SNP (single nucleotide polymorphism) markers associated with aflatoxin production were detected, and they explained 16.87%–31.70% of phenotypic variation (PVE), with SNP02686 and SNP19994 possessing 31.70% and 28.91% PVE, respectively. Aflatoxin contents of accessions with "AG" (existed in Zh.h0551 and Zh.h2150) and "GG" genotypes of either SNP19994 or SNP02686 were significantly lower than that of "AA" genotypes in the mean value of a three-year assay. The resistant accessions and molecular markers identified in this study are likely to be helpful for deployment in aflatoxin resistance breeding in peanuts. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20726651
Volume :
12
Issue :
3
Database :
Complementary Index
Journal :
Toxins
Publication Type :
Academic Journal
Accession number :
142524297
Full Text :
https://doi.org/10.3390/toxins12030156