Back to Search
Start Over
Succinate detection using in vivo 1H-MR spectroscopy identifies germline and somatic SDHx mutations in paragangliomas.
- Source :
- European Journal of Nuclear Medicine & Molecular Imaging; Jun2020, Vol. 47 Issue 6, p1510-1517, 8p, 1 Diagram, 2 Charts, 3 Graphs
- Publication Year :
- 2020
-
Abstract
- Purpose: Germline mutations in genes encoding succinate dehydrogenase (SDH) are frequent in patients with pheochromocytoma and paraganglioma (PPGL). They lead to SDH inactivation, mediating a massive accumulation of succinate, which constitutes a highly specific biomarker of SDHx-mutated tumors when measured in vitro. In a recent pilot study, we showed that magnetic resonance spectroscopy (<superscript>1</superscript>H-MRS) optimized for succinate detection (SUCCES) could detect succinate in vivo in both allografted mouse models and PPGL patients. The objective of this study was to prospectively assess the diagnostic performances of <superscript>1</superscript>H-MRS SUCCES sequence for the identification of SDH deficiency in PPGL patients. Methods: Forty-nine patients presenting with 50 PPGLs were prospectively enrolled in our referral center for <superscript>1</superscript>H-MRS SUCCES. Two observers blinded to the clinical characteristics and genetic status analyzed the presence of a succinate peak and confronted the results to a composite gold standard combining PPGL genetic testing and/or in vitro protein analyses in the tumor. Results: A succinate peak was observed in 20 tumors, all of which had proven SDH deficiency using the gold standard (17 patients with germline SDHx mutations, 2 with a somatic SDHD mutation, and 1 with negative SDHB IHC and SDH loss of function). A false negative result was observed in 3 tumors. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of <superscript>1</superscript>H-MRS SUCCES were respectively 87%, 100%, 100%, 90%, and 94%. Conclusions: Detection of succinate using <superscript>1</superscript>H-MRS is a highly specific and sensitive hallmark of SDH-deficiency in PPGLs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16197070
- Volume :
- 47
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- European Journal of Nuclear Medicine & Molecular Imaging
- Publication Type :
- Academic Journal
- Accession number :
- 142943502
- Full Text :
- https://doi.org/10.1007/s00259-019-04633-9