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Microfluidic device to study flow-free chemotaxis of swimming cells.
- Source :
- Lab on a Chip; 5/7/2020, Vol. 20 Issue 9, p1639-1647, 9p
- Publication Year :
- 2020
-
Abstract
- Microfluidic devices have been used in the last two decades to study in vitro cell chemotaxis, but few existing devices generate gradients in flow-free conditions. Flow can bias cell directionality of adherent cells and precludes the study of swimming cells like naïve T lymphocytes, which only migrate in a non-adherent fashion. We developed two devices that create stable, flow-free, diffusion-based gradients and are adapted for adherent and swimming cells. The flow-free environment is achieved by using agarose gel barriers between a central channel with cells and side channels with chemoattractants. These barriers insulate cells from injection/rinsing cycles of chemoattractants, they dampen residual drift across the device, and they allow co-culture of cells without physical interaction, to study contactless paracrine communication. Our devices were used here to investigate neutrophil and naïve T lymphocyte chemotaxis. [ABSTRACT FROM AUTHOR]
- Subjects :
- MICROFLUIDIC devices
CHEMOTAXIS
T cells
CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 14730197
- Volume :
- 20
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Lab on a Chip
- Publication Type :
- Academic Journal
- Accession number :
- 143082742
- Full Text :
- https://doi.org/10.1039/d0lc00045k