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Diagnosis of Parkinson's Disease by A Metabolomics-Based Laboratory-Developed Test (LDT).
- Source :
- Diagnostics (2075-4418); May2020, Vol. 10 Issue 5, p332, 1p
- Publication Year :
- 2020
-
Abstract
- A laboratory-developed test (LDT) is a type of in vitro diagnostic test that is designed, manufactured and used in the same laboratory (i.e., an in-house test). In this study, a metabolomics-based LDT was developed. This test involves a blood plasma preparation, direct-infusion mass spectrometry analysis with a high-resolution mass spectrometer, alignment and normalization of mass peaks using original algorithms, metabolite annotation by a biochemical context-driven algorithm, detection of overrepresented metabolic pathways and results in a visualization in the form of a pathway names cloud. The LDT was applied to detect early stage Parkinson's disease (PD)—the diagnosis of which currently requires great effort due to the lack of available laboratory tests. In a case–control study (n = 56), the LDT revealed a statistically sound pattern in the PD-relevant pathways. Usage of the LDT for individuals confirmed its ability to reveal this pattern and thus diagnose PD at the early-stage (1–2.5 stages, according to Hoehn and Yahr scale). The detection of this pattern by LDT could diagnose PD with a specificity of 64%, sensitivity of 86% and an accuracy of 75%. Thus, this LDT can be used for further widespread testing. [ABSTRACT FROM AUTHOR]
- Subjects :
- PARKINSON'S disease
MASS analysis (Spectrometry)
BLOOD plasma
MASS spectrometers
Subjects
Details
- Language :
- English
- ISSN :
- 20754418
- Volume :
- 10
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Diagnostics (2075-4418)
- Publication Type :
- Academic Journal
- Accession number :
- 144286753
- Full Text :
- https://doi.org/10.3390/diagnostics10050332