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Increase of BACE1, Brain-Renal Risk Factor, Contributes to Kidney Damage in an Alzheimer's Disease Mouse Model.
- Source :
- Journal of Alzheimer's Disease; 2020, Vol. 76 Issue 1, p237-248, 12p
- Publication Year :
- 2020
-
Abstract
- <bold>Background: </bold>It is believed that there is a certain correlation between the brain and kidneys, but it is poorly understood. Many findings suggested that there were previously unknown signaling pathways involving AβPP and BACE1 in the kidney.<bold>Objective: </bold>Exploring the changes of BACE1 activity in APP23 mouse kidneys, providing evidence for the function of AβPP and BACE1 activity in the kidney.<bold>Methods: </bold>The activity and expression of BACE1 were detected in the kidney of APP23 mice by enzymatic assay and western blotting. The protein expression levels of AβPP, claudin1, occludin, VE-cadherin, and Klotho (membrane-form klotho) were examined by using western blotting. The renal pathological changes of APP23 mice were examined by the routine renal pathological procedures.<bold>Results: </bold>In this study, we found that the AβPP protein level was increased in kidneys of APP23 mice compared with wild-type (WT) mice. Additionally, the activity and expression of BACE1 were increased in kidneys of APP23 mice compared to that of WT. BACE1 was predominantly distributed on the lumen side of renal tubular epithelial cells. The protein levels of Klotho and VE-cadherin were decreased, occludin expression was also decreased, and claudin-1 expression was increased. Renal pathological damage which observed in kidneys of APP23 mice was more serious than that in kidneys of WT mice.<bold>Conclusion: </bold>Our findings suggest that the increase of AβPP protein levels under Thy-1 neuron promoter in the APP23 mice promoted the increase of renal BACE1 expression and enzymatic activity in the kidneys. Moreover, certain pathological damage in the kidneys of APP23 mice were observed. APP23 mice are easily affected by external risk factors compared with WT mice. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROXIMAL kidney tubules
ALZHEIMER'S disease
MOUSE diseases
KIDNEYS
APOLIPOPROTEIN E4
RENAL tubular transport disorders
WESTERN immunoblotting
PROTEIN expression
BRAIN metabolism
BRAIN
RESEARCH
ANIMAL experimentation
RESEARCH methodology
PROTEIN precursors
PROTEOLYTIC enzymes
MEDICAL cooperation
EVALUATION research
COMPARATIVE studies
EPITHELIAL cells
MICE
Subjects
Details
- Language :
- English
- ISSN :
- 13872877
- Volume :
- 76
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Alzheimer's Disease
- Publication Type :
- Academic Journal
- Accession number :
- 144320527
- Full Text :
- https://doi.org/10.3233/JAD-200204