Back to Search
Start Over
Sevoflurane attenuates cardiomyocyte apoptosis by mediating the miR-219a/AIM2/TLR4/MyD88 axis in myocardial ischemia/reperfusion injury in mice.
- Source :
- Cell Cycle; Jul2020, Vol. 19 Issue 13, p1665-1676, 12p
- Publication Year :
- 2020
-
Abstract
- Myocardial infarction (MI) is a vital cause of death and disability globally. The primary treatment for diminishing acute myocardial ischemic injury is myocardial reperfusion, which may induce cardiomyocyte death. Our aim is to unravel the mechanism of sevoflurane (Sev) in microRNA-219a (miR-219a)-mediated regulation of absent in melanoma 2 (AIM2) and TLR4/MyD88 pathway during myocardial ischemia/reperfusion (I/R). The area of MI and apoptosis of cardiomyocytes of the developed mouse model were evaluated by TTC staining and TUNEL, respectively. After the determination of miR-219a as our target using microarray analysis, miR-219a atagomiR was used to treat the mouse model. The luciferase assay verified whether miR-219a targeted AIM2, and the miR-219a and AIM2 expression in myocardial tissues was detected by RT-qPCR and Western blot. miR-219a was significantly increased in myocardial tissues from mice treated with Sev, and the area of MI and cardiomyocyte apoptosis were decreased as a consequence. The miR-219a inhibitor reversed the action of Sev. Moreover, overexpression of AIM2 or induction of the TLR4 pathway aggravated myocardial I/R injury alleviated by miR-219a. All in all, the treatment of Sev upregulated miR-219a expression, which blocked the TLR4 pathway by targeting AIM2 and attenuated cardiomyocyte apoptosis in myocardial I/R mouse model. [ABSTRACT FROM AUTHOR]
- Subjects :
- APOPTOSIS
CORONARY disease
REPERFUSION injury
SEVOFLURANE
MYOCARDIAL reperfusion
Subjects
Details
- Language :
- English
- ISSN :
- 15384101
- Volume :
- 19
- Issue :
- 13
- Database :
- Complementary Index
- Journal :
- Cell Cycle
- Publication Type :
- Academic Journal
- Accession number :
- 144410837
- Full Text :
- https://doi.org/10.1080/15384101.2020.1765512