Ultrarapid diagnosis, microscope imaging, genome sequencing, and culture isolation of SARS-CoV-2.

Current technological level made possible the quick identification and culture of the SARS-CoV-2, an emerging coronavirus that caused until March 3, 2020, 90,870 confirmed cases of infections, with outside China a still limited spread (10,556 cases) and low mortality (166 deaths) (https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200303-sitrep-43-covid-19.pdf?sfvrsn=2c21c09c%5f2). This nasopharyngeal swab sample arrived at 8:30 pm in our clinical microbiology and virology laboratory at IHU Méditerranée Infection, which has been performing the routine diagnosis of SARS-CoV-2, 24 h a day, 7 days a week since the end of January 2020 for all suspected cases of Covid-19 (the SARS-CoV-2-associated disease). We carried out SARS-CoV-2 RNA detection by two first-line real-time reverse transcription (RT)-PCR assays [[9]], one of which (SpikeP ps80) was developed in-house as soon as the first SARS-CoV-2 genome (GenBank Accession no. [Extracted from the article]