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Ultrarapid diagnosis, microscope imaging, genome sequencing, and culture isolation of SARS-CoV-2.
- Source :
- European Journal of Clinical Microbiology & Infectious Diseases; Aug2020, Vol. 39 Issue 8, p1601-1603, 3p
- Publication Year :
- 2020
-
Abstract
- Current technological level made possible the quick identification and culture of the SARS-CoV-2, an emerging coronavirus that caused until March 3, 2020, 90,870 confirmed cases of infections, with outside China a still limited spread (10,556 cases) and low mortality (166 deaths) (https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200303-sitrep-43-covid-19.pdf?sfvrsn=2c21c09c%5f2). This nasopharyngeal swab sample arrived at 8:30 pm in our clinical microbiology and virology laboratory at IHU Méditerranée Infection, which has been performing the routine diagnosis of SARS-CoV-2, 24 h a day, 7 days a week since the end of January 2020 for all suspected cases of Covid-19 (the SARS-CoV-2-associated disease). We carried out SARS-CoV-2 RNA detection by two first-line real-time reverse transcription (RT)-PCR assays [[9]], one of which (SpikeP ps80) was developed in-house as soon as the first SARS-CoV-2 genome (GenBank Accession no. [Extracted from the article]
- Subjects :
- NUCLEOTIDE sequencing
DIAGNOSIS
AZITHROMYCIN
VIRUS diseases
MEDICAL microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 09349723
- Volume :
- 39
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- European Journal of Clinical Microbiology & Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 144457133
- Full Text :
- https://doi.org/10.1007/s10096-020-03869-w