Back to Search
Start Over
The Radiosensitizing Effect of Zinc Oxide Nanoparticles in Sub-Cytotoxic Dosing Is Associated with Oxidative Stress In Vitro.
- Source :
- Materials (1996-1944); Dec2019, Vol. 12 Issue 24, p4062, 1p, 6 Graphs
- Publication Year :
- 2019
-
Abstract
- Radioresistance is an important cause of head and neck cancer therapy failure. Zinc oxide nanoparticles (ZnO-NP) mediate tumor-selective toxic effects. The aim of this study was to evaluate the potential for radiosensitization of ZnO-NP. The dose-dependent cytotoxicity of ZnO-NP<subscript>20 nm</subscript> and ZnO-NP<subscript>100 nm</subscript> was investigated in FaDu and primary fibroblasts (FB) by an MTT assay. The clonogenic survival assay was used to evaluate the effects of ZnO-NP alone and in combination with irradiation on FB and FaDu. A formamidopyrimidine-DNA glycosylase (FPG)-modified single-cell microgel electrophoresis (comet) assay was applied to detect oxidative DNA damage in FB as a function of ZnO-NP and irradiation exposure. A significantly increased cytotoxicity after FaDu exposure to ZnO-NP<subscript>20 nm</subscript> or ZnO-NP<subscript>100 nm</subscript> was observed in a concentration of 10 µg/mL or 1 µg/mL respectively in 30 µg/mL of ZnO-NP<subscript>20 nm</subscript> or 20 µg/mL of ZnO-NP<subscript>100 nm</subscript> in FB. The addition of 1, 5, or 10 µg/mL ZnO-NP<subscript>20 nm</subscript> or ZnO-NP<subscript>100 nm</subscript> significantly reduced the clonogenic survival of FaDu after irradiation. The sub-cytotoxic dosage of ZnO-NP<subscript>100 nm</subscript> increased the oxidative DNA damage compared to the irradiated control. This effect was not significant for ZnO-NP<subscript>20 nm</subscript>. ZnO-NP showed radiosensitizing properties in the sub-cytotoxic dosage. At least for the ZnO-NP<subscript>100 nm</subscript>, an increased level of oxidative stress is a possible mechanism of the radiosensitizing effect. [ABSTRACT FROM AUTHOR]
- Subjects :
- OXIDATIVE stress
DNA damage
HEAD & neck cancer
NANOPARTICLES
CANCER treatment
Subjects
Details
- Language :
- English
- ISSN :
- 19961944
- Volume :
- 12
- Issue :
- 24
- Database :
- Complementary Index
- Journal :
- Materials (1996-1944)
- Publication Type :
- Academic Journal
- Accession number :
- 145132060
- Full Text :
- https://doi.org/10.3390/ma12244062