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Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells on the Acute Cigarette Smoke-Induced Pulmonary Inflammation Model.
- Source :
- Frontiers in Physiology; 8/12/2020, Vol. 11, pN.PAG-N.PAG, 11p
- Publication Year :
- 2020
-
Abstract
- Cigarette smoke (CS) has been reported to induce oxidative stress and inflammatory process in the lungs. However, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in the regulation of pulmonary inflammation remains unclear. The objective of this study is to investigate the effects of hUC-MSCs on lung inflammation in the acute CS-induced pulmonary inflammation animal model. Eight-week-old male C57BL/6 mice were intravenously administered 3 × 10<superscript>6</superscript>, 1 × 10<superscript>7</superscript>, and 3 × 10<superscript>7</superscript> cells/kg of hUC-MSCs as well as normal saline alone (control) after 3 days of CS exposure. Mice exposed to high-efficiency particulate air (HEPA)-filtered room air served as the CS control group. High-dose (3 × 10<superscript>7</superscript> cells/kg) hUC-MSC administration significantly decreased tumor necrosis factor (TNF)-α in the bronchoalveolar lavage fluid (BALF) of CS-exposed mice (p < 0.05). The chemokine (CXC motif) ligand 1/keratinocyte chemoattractant (CXCL1/KC) in BALF were significantly reduced by low-dose (3 × 10<superscript>6</superscript> cells/kg) and high-dose (3 × 10<superscript>7</superscript> cells/kg) hUC-MSC (p < 0.05). Medium-dose hUC-MSC administration decreased interleukin (IL)-1β in lung of mice, and TNF-α and caspase-3 were decreased in the lung of CS-exposed mice by medium- and high-dose MSC (p < 0.05). Low-dose hUC-MSCs significantly elevated serum CXCL1/KC and IL-1β in CS-exposed mice (p < 0.05). Our results suggest that high-dose hUC-MSCs reduced pulmonary inflammation and had antiapoptotic effects in acute pulmonary inflammation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1664042X
- Volume :
- 11
- Database :
- Complementary Index
- Journal :
- Frontiers in Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 145141896
- Full Text :
- https://doi.org/10.3389/fphys.2020.00962