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A Sensitive Isotope Modification of the Mixed Haemadsorption Test Applicable to the Study of Prozone Effects.
- Source :
- Immunology; Mar72, Vol. 22 Issue 3, p371-380, 10p
- Publication Year :
- 1972
-
Abstract
- Investigations of surface antigens of animal cells require sensitive and quantitative in vitro methods. A modification of the mixed haemadsorption technique (MHT) suitable for such purposes is presented. The indicator cells have been labelled with chromium-51. During studies of experimental conditions, including reproducibility and sensitivity, the technique was used in four model systems including histocompatibility (H-2, HL-A), species, organ and blood group antigens. Isotope labelling of the indicator cells has rendered the test more sensitive and in addition some qualitative properties of antigen-antibody interaction have been studied. The test procedure is simple and fast. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELLS
ANTIGENS
ANIMALS
IMMUNOGLOBULINS
IMMUNITY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 22
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14514658