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Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21.
- Source :
- Microchimica Acta; Aug2020, Vol. 187 Issue 8, pN.PAG-N.PAG, 1p
- Publication Year :
- 2020
-
Abstract
- A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F/F0 = 0.0031 × C<subscript>miRNA-21</subscript> + 1.0382 (R<superscript>2</superscript> = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice. [ABSTRACT FROM AUTHOR]
- Subjects :
- DNA
DNA probes
SUBSTITUTION reactions
DETECTION limit
MICRORNA
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 187
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 145258890
- Full Text :
- https://doi.org/10.1007/s00604-020-04377-6