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A wireless, implantable optoelectrochemical probe for optogenetic stimulation and dopamine detection.

Authors :
Liu, Changbo
Zhao, Yu
Cai, Xue
Xie, Yang
Wang, Taoyi
Cheng, Dali
Li, Lizhu
Li, Rongfeng
Deng, Yuping
Ding, He
Lv, Guoqing
Zhao, Guanlei
Liu, Lei
Zou, Guisheng
Feng, Meixin
Sun, Qian
Yin, Lan
Sheng, Xing
Source :
Microsystems & Nanoengineering; 8/242020, Vol. 6 Issue 1, pN.PAG-N.PAG, 1p
Publication Year :
2020

Abstract

Physical and chemical technologies have been continuously progressing advances in neuroscience research. The development of research tools for closed-loop control and monitoring neural activities in behaving animals is highly desirable. In this paper, we introduce a wirelessly operated, miniaturized microprobe system for optical interrogation and neurochemical sensing in the deep brain. Via epitaxial liftoff and transfer printing, microscale light-emitting diodes (micro-LEDs) as light sources and poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS)-coated diamond films as electrochemical sensors are vertically assembled to form implantable optoelectrochemical probes for real-time optogenetic stimulation and dopamine detection capabilities. A customized, lightweight circuit module is employed for untethered, remote signal control, and data acquisition. After the probe is injected into the ventral tegmental area (VTA) of freely behaving mice, in vivo experiments clearly demonstrate the utilities of the multifunctional optoelectrochemical microprobe system for optogenetic interference of place preferences and detection of dopamine release. The presented options for material and device integrations provide a practical route to simultaneous optical control and electrochemical sensing of complex nervous systems. Optogenetics: Simultaneous sensing and control A novel probe combines electrochemical sensing and optical control in a single wireless device, enabling researchers to remotely influence animal behavior and simultaneously measure their dopamine response. In optogenetic systems, light is used to switch on specific genetically-engineered neurons. A team led by Xing Sheng of China's Tsinghua University engineered a device with a microscale LED for optogenetic stimulation and an electrochemical sensor to detect dopamine. The two elements are separated by a diamond film for electrical and thermal insulation. The resulting device is roughly 2.2 × 1.3 cm and weighs 2 grams, including a miniaturized wireless circuit for control and a rechargeable battery for power. The team used the device to remotely train mice to prefer one chamber of a test environment via optogenetic stimulation and to measure animals' dopamine response. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20961030
Volume :
6
Issue :
1
Database :
Complementary Index
Journal :
Microsystems & Nanoengineering
Publication Type :
Academic Journal
Accession number :
145271655
Full Text :
https://doi.org/10.1038/s41378-020-0176-9