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Protein expression patterns in hek-Blue™ - Cells treated with Clinacanthus nutans extracts.
- Source :
- Pharmacognosy Magazine; 2020Supplement, Vol. 16 Issue 70, p431-434, 4p
- Publication Year :
- 2020
-
Abstract
- Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09731296
- Volume :
- 16
- Issue :
- 70
- Database :
- Complementary Index
- Journal :
- Pharmacognosy Magazine
- Publication Type :
- Academic Journal
- Accession number :
- 145470302
- Full Text :
- https://doi.org/10.4103/pm.pm_281_19