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Topoisomerase I Inhibition, DNA Photocleavage Activity, and G-Quadruplex DNA 'Light Switch' Based on Nitro-Substituted Ruthenium Complexes.
- Source :
- Russian Journal of Inorganic Chemistry; Aug2020, Vol. 65 Issue 8, p1186-1195, 10p
- Publication Year :
- 2020
-
Abstract
- Two ruthenium complexes containing nitro-substituted ligand, [Ru(bpy)<subscript>2</subscript>(hnpip)](PF<subscript>6</subscript>)<subscript>2</subscript> (1) and [Ru(phen)<subscript>2</subscript>(hnpip)](PF<subscript>6</subscript>)<subscript>2</subscript> (2) (bpy = 2,2'-bipyridine; phen = 1,10-phenanthroline; hnpip = 2-(3-hydroxyl- 4-nitrophenyl)imidazo[4,5-f][1,10-phenanthroline] were synthesized and characterized. DNA binding behaviors were studied by luminescent titration, UV-vis titration, FRET (FRET = Fluorescence resonance energy transfer) and molecular docking experiments. Complex 2 exhibited "light switch" effect upon addition of G-quadruplex DNA and displayed fluorescent selectivity towards G-quadruplex DNA over other DNAs in the presence of K<subscript>4</subscript>[Fe(CN)<subscript>6</subscript>]. Fluorescence experiments, FRET experiments and molecular docking results indicated that the selectivity may be attributed to protection of nitro group from water, DNA affinity and the difference in DNA structures. Furthermore, both complexes were found to cleave DNA under irradiation by the formation of singlet oxygen. DNA topoisomerase inhibition experiments indicated that complex 2 exhibited higher topoisomerase I inhibition activity (IC<subscript>50</subscript> = 18 μM) than complex 1 (IC<subscript>50</subscript> = 50 μM). Docking studies revealed that complex 2 stabilized Top1cc complex via π–π interaction and the formation of hydrogen bond. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00360236
- Volume :
- 65
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Russian Journal of Inorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 145675966
- Full Text :
- https://doi.org/10.1134/S0036023620080094