Back to Search
Start Over
Enhancement of protoplast preparation and regeneration of Hirsutella sinensis based on process optimization.
- Source :
- Biotechnology Letters; Nov2020, Vol. 42 Issue 11, p2357-2366, 10p
- Publication Year :
- 2020
-
Abstract
- Objective: To explore the optimal methods for the protoplast preparation and regeneration of Hirsutella sinensis by optimizing the limiting factors. Results: During the treatment of enzymatic protoplast preparation, mycelium cultured for 7 days was the optimal start material. The maximum protoplast preparation rate of 4.3 × 10<superscript>7</superscript> protoplasts/g fresh weight (FW) was obtained after 0.5 h treatment of 1 mg/ml mixed lytic enzymes in KH<subscript>2</subscript>PO<subscript>4</subscript>-K<subscript>2</subscript>HPO<subscript>4</subscript> buffer (pH 5.5) with 0.6 M KCl at 18 °C. As for the protoplast regeneration, the maximum protoplast regeneration rate reached 12.32% through 5 × 10<superscript>3</superscript> protoplasts mL<superscript>−1</superscript> cultivated for 20 days in the regeneration medium with 0.6 M mannitol and 1.5% agar. Conclusions: The preparation and regeneration of H. sinensis protoplasts was firstly established based on process optimization and it provided a foundation for the study of H. sinensis mutagenesis. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROCESS optimization
LYSINS
MANNITOL
PROTOPLASTS
MUTAGENESIS
AGAR
Subjects
Details
- Language :
- English
- ISSN :
- 01415492
- Volume :
- 42
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Biotechnology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 146105220
- Full Text :
- https://doi.org/10.1007/s10529-020-02958-2