Back to Search Start Over

Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment.

Authors :
Di Emidio, Giovanna
Rea, Francesco
Placidi, Martina
Rossi, Giulia
Cocciolone, Domenica
Virmani, Ashraf
Macchiarelli, Guido
Palmerini, Maria Grazia
D'Alessandro, Anna Maria
Artini, Paolo Giovanni
Tatone, Carla
Source :
Antioxidants; Sep2020, Vol. 9 Issue 9, p867, 1p
Publication Year :
2020

Abstract

Polycystic ovary syndrome (PCOS) is a complex metabolic disorder associated with female infertility. Based on energy and antioxidant regulatory functions of carnitines, we investigated whether acyl-L-carnitines improve PCOS phenotype in a mouse model induced by dehydroepiandrosterone (DHEA). CD1 mice received DHEA for 20 days along with two different carnitine formulations: one containing L-carnitine (LC) and acetyl-L-carnitine (ALC), and the other one containing also propionyl-L-carnitine (PLC). We evaluated estrous cyclicity, testosterone level, ovarian follicle health, ovulation rate and oocyte quality, collagen deposition, lipid droplets, and 17ß-HSD IV (17 beta-hydroxysteroid dehydrogenase type IV) expression. Moreover, we analyzed protein expression of SIRT1, SIRT3, SOD2 (superoxide dismutase 2), mitochondrial transcriptional factor A (mtTFA), RAGE (receptor for AGEs), GLO2 (glyoxalase 2) and ovarian accumulation of MG-AGEs (advanced glycation end-products formed by methylglyoxal). Both carnitine formulations ameliorated ovarian PCOS phenotype and positively modulated antioxidant molecular pathways in the ovarian microenvironment. Addition of PLC to LC-ALC formulation mitigated intraovarian MG-AGE accumulation and increased mtTFA expression. In conclusion, our study supports the hypothesis that oral administration of acyl-L-carnitines alleviates ovarian dysfunctions associated with this syndrome and that co-administration of PLC provides better activity. Molecular mechanisms underlying these effects include anti-oxidant/glycative activity and potentiation of mitochondria. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20763921
Volume :
9
Issue :
9
Database :
Complementary Index
Journal :
Antioxidants
Publication Type :
Academic Journal
Accession number :
146143483
Full Text :
https://doi.org/10.3390/antiox9090867