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Cloning of Genes Sef1 and Tup1 Encoding Transcriptional Activator and Global Repressor in the Flavinogenic Yeast Meyerozyma (Candida, Pichia) guilliermondii.

Authors :
Fedorovych, D.
Boretsky, V.
Pynyaha, Y.
Bohovych, I.
Boretsky, Y.
Sibirny, A.
Source :
Cytology & Genetics; Sep2020, Vol. 54 Issue 5, p413-419, 7p
Publication Year :
2020

Abstract

Abstract—Two Meyerozyma (Candida, Pichia) guilliermondii genes coding for homologs of transcriptional factor Sef1p of Candida famata and Tup1p of Candida albicans were identified, cloned, and deleted. Deletion of a homologue of Sef1p transcriptional factor in M.(P.) guilliermondii completely blocked over-synthesis of riboflavin under conditions of iron deficiency. The results of genetic complementation analysis suggest that previously reported rib83 mutants and newly constructed knock-out strains belong to the same complementation group and are defective in the same SEF1 gene. Inactivation of the identified homolog of the TUP1 gene in M.(P.) guilliermondii wild-type strain led to 1.5-fold increase in cellular iron content and 1.5–1.7-fold increase in riboflavin production. Introduction of a plasmid-borne copy of the TUP1 gene did not restore metabolic defects of the riboflavin overproduction and iron accumulation in mutant strain M. (P.) guilliermondii m3, bearing the hit1 mutation. The obtained results suggest that both transcription factors Sef1p and Tup1p are involved in the regulation of iron acquisition and riboflavin biosynthesis by yeast belonging to the CUG-clade. The molecular mechanism of action Tup1p on riboflavin biosynthesis in M.(P.) guilliermondii required further elucidation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00954527
Volume :
54
Issue :
5
Database :
Complementary Index
Journal :
Cytology & Genetics
Publication Type :
Academic Journal
Accession number :
146390158
Full Text :
https://doi.org/10.3103/S0095452720050072