Back to Search Start Over

Identification of a novel prognostic DNA methylation signature for lung adenocarcinoma based on consensus clustering method.

Authors :
Cai, Qidong
He, Boxue
Xie, Hui
Zhang, Pengfei
Peng, Xiong
Zhang, Yuqian
Zhao, Zhenyu
Wang, Xiang
Source :
Cancer Medicine; Oct2020, Vol. 9 Issue 20, p7488-7502, 15p
Publication Year :
2020

Abstract

Abnormal DNA methylation persists throughout carcinogenesis and cancer development. Hence, gene promoter methylation may act as a prognostic tool and provide new potential therapeutic targets for patients with lung adenocarcinoma (LUAD). In this study, to explore prognostic methylation signature, data regarding DNA methylation and RNA‐seq, and clinical data of patients with LUAD from the Cancer Genome Atlas database (TCGA) were downloaded. After data preprocessing, the methylation data were divided into training (N = 405) and test sets (N = 62). Then, patients in the training set were assigned to five subgroups based on their different methylation levels using the consensus clustering method. We comprehensively analyzed the survival information, methylation levels, and clinical variables, including American Joint Committee on Cancer (AJCC) stage, tumor‐node‐metastasis (TNM) staging, age, smoking history, and gender of these five groups. Subsequently, we identified a 16‐CpG prognostic signature and constructed a prognostic model, which was verified in the test set. Further analyses showed stable prognostic performance in the stratified cohorts. In conclusion, the new predictive DNA methylation signature proposed in this study may be used as an independent biomarker to assess the overall survival of LUAD patients and provide bioinformatics information for development of targeted therapy. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20457634
Volume :
9
Issue :
20
Database :
Complementary Index
Journal :
Cancer Medicine
Publication Type :
Academic Journal
Accession number :
146508031
Full Text :
https://doi.org/10.1002/cam4.3343