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ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans.
- Source :
- Frontiers in Physiology; 10/23/2020, Vol. 11, pN.PAG-N.PAG, 14p
- Publication Year :
- 2020
-
Abstract
- As part of the ZOOMICS project, we set out to investigate common and diverging metabolic traits in the blood metabolome across various species by taking advantage of recent developments in high-throughput metabolomics. Here we provide the first comparative metabolomics analysis of fresh and stored human (n = 21, 10 males, 11 females), olive baboon (n = 20), and rhesus macaque (n = 20) red blood cells at baseline and upon 42 days of storage under blood bank conditions. The results indicated similarities and differences across species, which ultimately resulted in a differential propensity to undergo morphological alterations and lyse as a function of the duration of refrigerated storage. Focusing on purine oxidation, carboxylic acid, fatty acid, and arginine metabolism further highlighted species-specific metabolic wiring. For example, through a combination of steady state measurements and <superscript>13</superscript>C<subscript>6</subscript><superscript>15</superscript>N<subscript>4</subscript>-arginine tracing experiments, we report an increase in arginine catabolism into ornithine in humans, suggestive of species-specific arginase 1 activity and nitric oxide synthesis—an observation that may impact the translatability of cardiovascular disease studies carried out in non-human primates (NHPs). Finally, we correlated metabolic measurements to storage-induced morphological alterations via scanning electron microscopy and hemolysis, which were significantly lower in human red cells compared to both NHPs. [ABSTRACT FROM AUTHOR]
- Subjects :
- CERCOPITHECIDAE
ERYTHROCYTES
METABOLOMICS
RHESUS monkeys
COMPARATIVE biology
Subjects
Details
- Language :
- English
- ISSN :
- 1664042X
- Volume :
- 11
- Database :
- Complementary Index
- Journal :
- Frontiers in Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 146629982
- Full Text :
- https://doi.org/10.3389/fphys.2020.593841