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Small grain and semi-dwarf 3, a WRKY transcription factor, negatively regulates plant height and grain size by stabilizing SLR1 expression in rice.

Authors :
Lan, Jie
Lin, Qibing
Zhou, Chunlei
Ren, Yakun
Liu, Xi
Miao, Rong
Jing, Ruonan
Mou, Changling
Nguyen, Thanhliem
Zhu, Xingjie
Wang, Qian
Zhang, Xin
Guo, Xiuping
Liu, Shijia
Jiang, Ling
Wan, Jianmin
Source :
Plant Molecular Biology; Nov2020, Vol. 104 Issue 4/5, p429-450, 22p
Publication Year :
2020

Abstract

Key message: OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and grain size. WRKYs are transcription factors that regulate stress tolerance and plant development including height and grain size. However, the relationship between GA signaling and WRKY genes is still poorly understood. Here, we characterized a small grain and semi-dwarf 3 (sgsd3) mutant in rice cv. Hwayoung (WT). A T-DNA insertion in the 5′-UTR of OsWRKY36 induced overexpression of OsWRKY36 in the sgsd3 mutant, likely leading to the mutant phenotype. This was confirmed by the finding that overexpression of OsWRKY36 caused a similar small grain and semi-dwarf phenotype to the sgsd3 mutant whereas knock down and knock out caused larger grain phenotypes. The sgsd3 mutant was also hyposensitive to GA and accumulated higher mRNA and protein levels of SLR1 (a GA signaling DELLA-like inhibitor) compared with the WT. Further assays showed that OsWRKY36 enhanced SLR1 transcription by directly binding to its promoter. In addition, we found that OsWRKY36 can protect SLR1 from GA-mediated degradation. We thus identified a new GA signaling repressor OsWRKY36 that represses GA signaling through stabilizing the expression of SLR1. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01674412
Volume :
104
Issue :
4/5
Database :
Complementary Index
Journal :
Plant Molecular Biology
Publication Type :
Academic Journal
Accession number :
146680055
Full Text :
https://doi.org/10.1007/s11103-020-01049-0