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In-House Developed ELISA Indicates High Prevalence of Anti- Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan.
- Source :
- Pathogens; Nov2020, Vol. 9 Issue 11, p905-905, 1p
- Publication Year :
- 2020
-
Abstract
- Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using probability proportional to size (PPS) statistical technique. Epidemiological information was recorded on a questionnaire for the estimation of potential risk factors. The serum samples were analyzed for IgG antibodies against Echinococcus granulosus using an in-house-developed EgAgB-based ELISA kit. The overall seroprevalence recorded was 21.98% (160/728) in the tested sheep, suggesting higher seropositivity in sheep from Punjab (23.73%) as compared to Khyber Pakhtunkhwa (KPK) (19.04%). The overall apparent prevalence observed by this ELISA method was almost similar to the calculated true prevalence (21.77%). Prevalence was significantly different (p < 0.05) among sheep from different districts. Higher prevalence was found in females (22.54%, OR 1.41), age group > 5 years (29.66%, OR 1.64), crossbreeds (42.85%, OR 2.70), and sheep with pasture access (25.96%, OR 3.06). Being in age group > 5 years and having pasture access were the factors significantly associated with seropositivity (p < 0.05). This study provides serological evidence of E. granulosus infection in sheep and can be used as a model for ante-mortem screening of the sheep globally. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 9
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 147273198
- Full Text :
- https://doi.org/10.3390/pathogens9110905