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Development of an immunocolloidal strip for rapid detection of picoxystrobin.

Authors :
Song, Shanshan
Chen, Ziwen
Liu, Jie
Xu, Liguang
Kuang, Hua
Zhu, Jianping
Liu, Liqiang
Source :
Food & Agricultural Immunology; Dec2020, Vol. 31 Issue 1, p711-722, 12p
Publication Year :
2020

Abstract

In this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC, and its IC<subscript>50</subscript> value was 0.1 ng/ml. The monoclonal antibody was then used to prepare immunochromatographic strips, and its cut-off values in both phosphate-buffered saline solution and cucumber samples were 50 and 100 ng/ml, respectively. It was concluded that the ICS can sensitively and rapidly detect PIC in cucumber samples. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09540105
Volume :
31
Issue :
1
Database :
Complementary Index
Journal :
Food & Agricultural Immunology
Publication Type :
Academic Journal
Accession number :
147528203
Full Text :
https://doi.org/10.1080/09540105.2020.1754344