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Structure of the Dietzia Mrp complex reveals molecular mechanism of this giant bacterial sodium proton pump.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; 12/8/2020, Vol. 117 Issue 49, p31166-31176, 11p
- Publication Year :
- 2020
-
Abstract
- Multiple resistance and pH adaptation (Mrp) complexes are sophisticated cation/proton exchangers found in a vast variety of alkaliphilic and/or halophilic microorganisms, and are critical for their survival in highly challenging environments. This family of antiporters is likely to represent the ancestor of cation pumps found in many redox-driven transporter complexes, including the complex I of the respiratory chain. Here, we present the threedimensional structure of the Mrp complex from a Dietzia sp. strain solved at 3.0-Å resolution using the single-particle cryoelectron microscopy method. Our structure-based mutagenesis and functional analyses suggest that the substrate translocation pathways for the driving substance protons and the substrate sodium ions are separated in two modules and that symmetry-restrained conformational change underlies the functional cycle of the transporter. Our findings shed light on mechanisms of redox-driven primary active transporters, and explain how driving substances of different electric charges may drive similar transport processes. [ABSTRACT FROM AUTHOR]
- Subjects :
- ELECTRIC charge
PROTONS
SODIUM ions
HALOPHILIC microorganisms
SODIUM compounds
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 117
- Issue :
- 49
- Database :
- Complementary Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 147572947
- Full Text :
- https://doi.org/10.1073/pnas.2006276117