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Up-Regulation of GATA4 Regulates Human Lens Epithelial Cell Function in Age-Related Cataract.
- Source :
- Ophthalmic Research; 2020, Vol. 63 Issue 6, p564-571, 8p
- Publication Year :
- 2020
-
Abstract
- Purpose: GATA4 has emerged as a novel regulator that plays a critical role in mediating senescence. However, the role of GATA4 in age-related cataract (ARC), the leading cause of visual impairment, requires further elucidation. Methods: GATA4 expression was measured by quantitative RT-PCR and capillary Western immunoassay (WES). The MTT assay, EdU assay, and rhodamine-123/Hoechst and calcein-AM/propidium iodide double staining were used to investigate the role of GATA4 in the viability, proliferation, and apoptosis of cultured human lens epithelial cells (HLECs). Results: HLECs were subjected to 3 different treatment models, including prolonged exposure to low-dose H<subscript>2</subscript>O<subscript>2</subscript>, UVB irradiation, and mild heating, to simulate senescence and apoptosis. GATA4 expression was significantly increased in these models in a time- and dose-dependent manner. Overexpression of GATA4 reduced cell viability, accelerated apoptosis development, and reduced the proliferation of HLECs. Furthermore, the expression of GATA4 from ARC was up-regulated at both mRNA and at protein level compared with clear lenses. Conclusion: GATA4 is up-regulated in all 3 models of HLECs in vitro and the cells from ARC lenses in vivo. Up-regulation of GATA4 mediates HLEC dysfunction. GATA4-mediated effects in HLECs would provide a novel insight into the pathogenesis of ARC. [ABSTRACT FROM AUTHOR]
- Subjects :
- EPITHELIAL cells
CELL physiology
CATARACT
APOPTOSIS
VISION disorders
Subjects
Details
- Language :
- English
- ISSN :
- 00303747
- Volume :
- 63
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Ophthalmic Research
- Publication Type :
- Academic Journal
- Accession number :
- 147671155
- Full Text :
- https://doi.org/10.1159/000507962