Application of Polyacrylamide Flocculant for Stabilization of Anaerobic Digestion under Conditions of Excessive Accumulation of Volatile Fatty Acids.

Featured Application: Here we establish a strategy for recovering the methanogenic activity in acidified reactors by PAM-forced flocculation. Excessive accumulation of volatile fatty acids (VFA) is one of the major factors destabilizing methanogenic digestion of organic wastes in anaerobic bioreactors. Existing methods of stabilization of this process are mostly expensive and labor-intensive, often requiring removal of a considerable portion of acidified biomass from the bioreactor. We propose a method for methanogenesis restoration in such soured reactors by the addition of a cationic polyacrylamide flocculant (PAM) at 20 mg/g total solids. After flocculant addition, mixing should be minimized to prolong the existence of the floccules formed in the presence of the flocculant. While partial microbial degradation of the polyacrylamide flocculant was observed during the thermophilic anaerobic process, complete PAM mineralization did not occur. Significant inhibition of anaerobic processes, primarily in the activity of syntrophic propionate-oxidizing bacteria, was observed at PAM concentrations above 40 mg/g total solids. [ABSTRACT FROM AUTHOR]