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Comparison of Four SARS-CoV-2 Neutralization Assays.
- Source :
- Vaccines; Jan2021, Vol. 9 Issue 1, p13, 1p
- Publication Year :
- 2021
-
Abstract
- Neutralizing antibodies are a major correlate of protection for many viruses including the novel coronavirus SARS-CoV-2. Thus, vaccine candidates should potently induce neutralizing antibodies to render effective protection from infection. A variety of in vitro assays for the detection of SARS-CoV-2 neutralizing antibodies has been described. However, validation of the different assays against each other is important to allow comparison of different studies. Here, we compared four different SARS-CoV-2 neutralization assays using the same set of patient samples. Two assays used replication competent SARS-CoV-2, a focus forming assay and a TCID<subscript>50</subscript>-based assay, while the other two assays used replication defective lentiviral or vesicular stomatitis virus (VSV)-based particles pseudotyped with SARS-CoV-2 spike. All assays were robust and produced highly reproducible neutralization titers. Titers of neutralizing antibodies correlated well between the different assays and with the titers of SARS-CoV-2 S-protein binding antibodies detected in an ELISA. Our study showed that commonly used SARS-CoV-2 neutralization assays are robust and that results obtained with different assays are comparable. [ABSTRACT FROM AUTHOR]
- Subjects :
- SARS-CoV-2
VESICULAR stomatitis
VITRONECTIN
ANTIBODY titer
Subjects
Details
- Language :
- English
- ISSN :
- 2076393X
- Volume :
- 9
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Vaccines
- Publication Type :
- Academic Journal
- Accession number :
- 148287112
- Full Text :
- https://doi.org/10.3390/vaccines9010013