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High-Specific Fluorescence Probe for SO32− Detection and Bioimaging.
- Source :
- Journal of Fluorescence; Mar2021, Vol. 31 Issue 2, p363-371, 9p
- Publication Year :
- 2021
-
Abstract
- It is well known that sulfite (SO<subscript>3</subscript><superscript>2−</superscript>) plays an indispensable role in various physiological processes. Abnormal levels of SO<subscript>3</subscript><superscript>2−</superscript> can trigger a wide variety of diseases involving respiratory, nervous and cardiovascular systems. Hence, it is necessary to find an efficient approach for detection of SO<subscript>3</subscript><superscript>2−</superscript>. In this study, a pyrene derivative, (E)-4-(3-oxo-3-(pyren-1-yl)prop-1-en-1-yl)phenyl acrylate (PPA), was designed and synthesized for monitoring SO<subscript>3</subscript><superscript>2−</superscript>. The probe possessed simple synthetic steps, excellent anti-interference ability and specific response to SO<subscript>3</subscript><superscript>2−</superscript> in the presence of other substances. The reaction between PPA and SO<subscript>3</subscript><superscript>2−</superscript> was ascribed to Michael addition and the detection mechanism was confirmed by HRMS spectra analysis and FTIR analysis. Additionally, PPA responded linearly to detect SO<subscript>3</subscript><superscript>2−</superscript> within the rang of 0–100 μM. The limit of detection was calculated as low as 0.17 μM in accordance with the recommendation of IUPAC (CDL =3sb/m). Notably, PPA was further applied in biological imaging in HepG2 cells, which provided a possibility to monitor SO<subscript>3</subscript><superscript>2−</superscript> in vivo. [ABSTRACT FROM AUTHOR]
- Subjects :
- PYRENE derivatives
FLUORESCENCE
SPECTRUM analysis
CELL imaging
NERVOUS system
Subjects
Details
- Language :
- English
- ISSN :
- 10530509
- Volume :
- 31
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Journal of Fluorescence
- Publication Type :
- Academic Journal
- Accession number :
- 148802527
- Full Text :
- https://doi.org/10.1007/s10895-020-02662-4