Back to Search
Start Over
mTORC1‐mediated amino acid signaling is critical for cell fate determination under transplant‐induced stress.
- Source :
- FEBS Letters; Feb2021, Vol. 595 Issue 4, p462-475, 14p
- Publication Year :
- 2021
-
Abstract
- Transplantation of in vitro‐manipulated cells is widely used in hematology. While transplantation is well recognized to impose severe stress on transplanted cells, the nature of transplant‐induced stress remains elusive. Here, we propose that the lack of amino acids in serum is the major cause of transplant‐induced stress. Mechanistically, amino acid deficiency decreases protein synthesis and nutrient consummation. However, in cells with overactive AKT and ERK, mTORC1 is not inhibited and protein synthesis remains relatively high. This impaired signaling causes nutrient depletion, cell cycle block, and eventually autophagy and cell death, which can be inhibited by cycloheximide or mTORC1 inhibitors. Thus, mTORC1‐mediated amino acid signaling is critical in cell fate determination under transplant‐induced stress, and protein synthesis inhibition can improve transplantation efficiency. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL determination
AMINO acids
PROTEIN synthesis
CELL death
CELL cycle
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 595
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 148862311
- Full Text :
- https://doi.org/10.1002/1873-3468.14008