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Cathodic photoelectrochemical aptasensor based on NiO/BiOI/Au NP composite sensitized with CdSe for determination of exosomes.
- Source :
- Microchimica Acta; Feb2021, Vol. 188 Issue 2, p1-10, 10p
- Publication Year :
- 2021
-
Abstract
- A cathodic photoelectrochemical sensor has been developed for the determination of exosomes, based on a dual-signal reduction strategy. A heterostructure of NiO/BiOI/Au NP/CdSe was synthesized as a photoelectrochemical sensing interface, which is able to suppress the recombination of electron-hole pairs and produce a higher photocurrent. The obtained materials were characterized, and the mechanism for the generation of the cathodic photocurrent was proposed. CdSe QDs (quantum dots) modified with DNA<subscript>2</subscript> were assembled on the electrode through the hybridization with EpCAM aptamer on the surface of ITO/NiO/BiOI/Au NP. The introduction of CdSe QDs to the electrode increases the photocurrent.The recognition of exosomes with aptamer DNA led to the separation of CdSe QDs from the electrode, which in turn caused the decrease of photocurrents. Meanwhile, the big volume of exosomes hinders the electron transfer between the electrode and electrolyte. Due to the dual reduction effect, a sensitive PEC sensor was obtained with a detection limit of 1.2 × 10<superscript>2</superscript> particles/μL exosomes (λ<subscript>ex</subscript> = 430 nm, bias voltage = − 0.1 V). The cathodic photoelectrochemical sensor showed good selectivity, performed well in a complex biological environment and could be used to distinguishbreast cancer patients from healthy individuals. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 188
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 148888534
- Full Text :
- https://doi.org/10.1007/s00604-021-04716-1