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CTP‐CM enhances osteogenic differentiation of hPDLSCs via NF‐κB pathway.
- Source :
- Oral Diseases; Apr2021, Vol. 27 Issue 3, p577-588, 12p, 5 Color Photographs, 2 Charts
- Publication Year :
- 2021
-
Abstract
- Objective: The conditioned medium of calcined tooth powder (CTP‐CM) is a type of biomimetic mineralized material and well contributing to bone healing and bone formation in vivo. However, little is known about the effect of CTP‐CM on human periodontal ligament stem cells (hPDLSCs) as well as the underlying mechanisms. Methods: ALP activity assay was conducted to select the concentration with the highest ALP level, which was used for the following experiments. Cell proliferation was measured by cell counting kit‐8 assay and flow cytometry analysis. Expression levels of osteogenic markers in CTP‐CM‐induced hPDLSCs were evaluated with real‐time quantitative reverse transcription polymerase chain reaction (qRT‐PCR), immunofluorescence staining, and Western blot. Mineralization of CTP‐CM‐induced hPDLSCs was evaluated by alizarin red staining. Furthermore, the involvement of NF‐κB pathway was examined by immunofluorescence staining and Western blot. Results: 20 μg/ml was selected for the further experiments. Functional studies demonstrated that CTP‐CM exerted almost no influence on the proliferation of hPDLSCs and CTP‐CM increased the osteogenic differentiation of hPDLSCs. Mechanistically, CTP‐CM leads to activation of NF‐κB signaling pathway. When treated with BMS345541, the osteogenic differentiation of CTP‐CM‐treated hPDLSCs was significantly attenuated. Conclusion: CTP‐CM can promote the osteogenic differentiation of hPDLSCs via activating NF‐κB pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL differentiation
FLOW cytometry
REVERSE transcriptase polymerase chain reaction
BONE growth
IN vivo studies
STAINS & staining (Microscopy)
WESTERN immunoblotting
CELLULAR signal transduction
GENE expression
TREATMENT effectiveness
POLYMERS
DNA-binding proteins
STEM cells
CELL proliferation
FLUORESCENT antibody technique
POLYMERASE chain reaction
BONE density
PERIODONTAL ligament
FRACTURE healing
Subjects
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 27
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 148997272
- Full Text :
- https://doi.org/10.1111/odi.13567