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Precise identification of an RNA methyltransferase's substrate modification site.
- Source :
- Chemical Communications; 3/11/2021, Vol. 57 Issue 20, p2499-2502, 4p
- Publication Year :
- 2021
-
Abstract
- Here we report a simple and nonradioactive biochemical assay which is capable of accurately determining the substrate methylation sites of human RNA N<superscript>6</superscript>-methyladenosine methyltransferases METTL3/METTL14 and METTL16. This method employs enzyme-assisted chemical labelling of a specific base in an RNA substrate with the assistance of an allyl-substituted methyltransferase cofactor, and enables precise identification of the labelling site by a mutation signal from standard nucleic acid sequencing. Our method provides a platform to investigate the enzymatic methylations of long and structurally complex RNA substrates, and facilitates the discovery of new methyltransferases. [ABSTRACT FROM AUTHOR]
- Subjects :
- RNA
METHYLTRANSFERASES
CHEMICAL labeling
NUCLEIC acids
METHYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 57
- Issue :
- 20
- Database :
- Complementary Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 149146968
- Full Text :
- https://doi.org/10.1039/d0cc08260k