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Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19.
- Source :
- International Journal of Molecular Sciences; Mar2021, Vol. 22 Issue 5, p2723-2723, 1p
- Publication Year :
- 2021
-
Abstract
- Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralization assays. The serology assays test the IgG and IgM against both the full-length spike antigens and the receptor binding domain (RBD) of the spike antigen. Benchmarking against an RBD-specific SARS-CoV IgG reference standard, the anti-SARS-CoV-2 RBD antibody titer was quantified in the range of 37.6 µg/mL to 31.0 ng/mL. The quantitative assays are highly specific with no correlative cross-reactivity with the spike proteins of MERS, SARS1, OC43 and HKU1 viruses. We further demonstrated good correlation between anti-RBD antibody titers and neutralizing antibody titers. The suite of serology and neutralization assays help to improve measurement confidence and are complementary and foundational for clinical and epidemiologic studies. [ABSTRACT FROM AUTHOR]
- Subjects :
- COVID-19
COVID-19 pandemic
ANTIBODY titer
ANTIGEN receptors
SEROLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 22
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 149241058
- Full Text :
- https://doi.org/10.3390/ijms22052723