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Transcriptome analysis of cepharanthine against a SARS-CoV-2-related coronavirus.
- Source :
- Briefings in Bioinformatics; Mar2021, Vol. 22 Issue 2, p1378-1386, 9p
- Publication Year :
- 2021
-
Abstract
- Antiviral therapies targeting the pandemic coronavirus disease 2019 (COVID-19) are urgently required. We studied an already-approved botanical drug cepharanthine (CEP) in a cell culture model of GX_P2V, a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-related virus. RNA-sequencing results showed the virus perturbed the expression of multiple genes including those associated with cellular stress responses such as endoplasmic reticulum (ER) stress and heat shock factor 1 (HSF1)-mediated heat shock response, of which heat shock response-related genes and pathways were at the core. CEP was potent to reverse most dysregulated genes and pathways in infected cells including ER stress/unfolded protein response and HSF1-mediated heat shock response. Additionally, single-cell transcriptomes also confirmed that genes of cellular stress responses and autophagy pathways were enriched in several peripheral blood mononuclear cells populations from COVID-19 patients. In summary, this study uncovered the transcriptome of a SARS-CoV-2-related coronavirus infection model and anti-viral activities of CEP, providing evidence for CEP as a promising therapeutic option for SARS-CoV-2 infection. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14675463
- Volume :
- 22
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Briefings in Bioinformatics
- Publication Type :
- Academic Journal
- Accession number :
- 149507155
- Full Text :
- https://doi.org/10.1093/bib/bbaa387