Single‐cell analysis reveals innate immunity dynamics in ankylosing spondylitis.

Taken together, the percentages of MS2 cells were decreased in AS patients and might be recovered by TNF- blockers treatment in the good responders but not the poor responders, suggesting that MS2 cell proportion could be a potential indicator to the response to TNF- blocker treatment and MS2 cells might be beneficial for AS treatment. Data of NS5 are shown in Supporting Information figures gl In conclusion, we profiled the transcriptional landscape of monocytes and NK cells, which are two pivotal cell types of innate immunity in AS at single-cell resolution. Monocytes and NK cells were isolated from peripheral blood mononuclear cells using magnetic beads with high specificity (Supporting Information). Notably, the proportion of NS2 cells (FCER1G SP - sp FCGR3A SP + sp NK cells, similar to memory-like NK cells) differed significantly between AS patients and healthy controls and might serve as a potential indicator for AS (Figure 3C). [Extracted from the article]