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Astaxanthin as a new Raman probe for biosensing of specific subcellular lipidic structures: can we detect lipids in cells under resonance conditions?

Authors :
Czamara, Krzysztof
Adamczyk, Adriana
Stojak, Marta
Radwan, Basseem
Baranska, Malgorzata
Source :
Cellular & Molecular Life Sciences; Apr2021, Vol. 78 Issue 7, p3477-3484, 8p
Publication Year :
2021

Abstract

Here we report a new Raman probe for cellular studies on lipids detection and distribution. It is (3S, 3'S)-astaxanthin (AXT), a natural xanthophyll of hydrophobic properties and high solubility in lipids. It contains a chromophore group, a long polyene chain of eleven conjugated C=C bonds including two in the terminal rings, absorbing light in the visible range that coincides with the excitation of lasers commonly used in Raman spectroscopy for studying of biological samples. Depending on the laser, resonance (excitation in the visible range) or pre-resonance (the near infrared range) Raman spectrum of astaxanthin is dominated by bands at ca. 1008, 1158, and 1520 cm<superscript>−1</superscript> that now can be also a marker of lipids distribution in the cells. We showed that AXT accumulates in lipidic structures of endothelial cells in time-dependent manner that provides possibility to visualize e.g. endoplasmic reticulum, as well as nuclear envelope. As a non-toxic reporter, it has a potential in the future studies on e.g. nucleus membranes damage in live cells in a very short measuring time. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1420682X
Volume :
78
Issue :
7
Database :
Complementary Index
Journal :
Cellular & Molecular Life Sciences
Publication Type :
Academic Journal
Accession number :
149761545
Full Text :
https://doi.org/10.1007/s00018-020-03718-1