Validation of a colorimetric method for determination of paracetamol in exhaled breath condensate.

A redox reaction-based colorimetric method was validated for the qualitative assay and quantitative determination of paracetamol in exhaled breath condensate (EBC). Fe (III) and K₃[Fe(CN)₆] were used as colorimetric reagents in which paracetamol reduces Fe (III) to Fe (II) due to its reducing property. The produced Fe (II) is chelated with K₃[Fe(CN)₆] to form prussian blue colored product which can be detected by the naked eye in the range of 0.2–10.0 μg mL⁻¹. The fully validated method was used for the determination of paracetamol in EBC of paracetamol poisoning individuals. [ABSTRACT FROM AUTHOR]