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Transcriptome of Tumor-Infiltrating T Cells in Colorectal Cancer Patients Uncovered a Unique Gene Signature in CD4 + T Cells Associated with Poor Disease-Specific Survival.
- Source :
- Vaccines; Apr2021, Vol. 9 Issue 4, p334, 1p
- Publication Year :
- 2021
-
Abstract
- Colorectal cancer (CRC) is influenced by infiltration of immune cell populations in the tumor microenvironment. While elevated levels of cytotoxic T cells are associated with improved prognosis, limited studies have reported associations between CD4<superscript>+</superscript> T cells and disease outcomes. We recently performed transcriptomic profiling and comparative analyses of sorted CD4<superscript>+</superscript> and CD8<superscript>+</superscript> tumor-infiltrating lymphocytes (TILs) from bulk tumors of CRC patients with varying disease stages. In this study, we compared the transcriptomes of CD4<superscript>+</superscript> with CD8<superscript>+</superscript> TILs. Functional annotation pathway analyses revealed the downregulation of inflammatory response-related genes, while T cell activation and angiogenesis-related genes were upregulated in CD4<superscript>+</superscript> TILs. The top 200 deregulated genes in CD4<superscript>+</superscript> TILs were aligned with the cancer genome atlas (TCGA) CRC dataset to identify a unique gene signature associated with poor prognosis. Moreover, 69 upregulated and 20 downregulated genes showed similar trends of up/downregulation in the TCGA dataset and were used to calculate "poor prognosis score" (ppScore), which was significantly associated with disease-specific survival. High ppScore patients showed lower expression of Treg-, Th1-, and Th17-related genes, and higher expression of Th2-related genes. Our data highlight the significance of T cells within the TME and identify a unique candidate prognostic gene signature for CD4<superscript>+</superscript> TILs in CRC patients. [ABSTRACT FROM AUTHOR]
- Subjects :
- COLORECTAL cancer
T cells
CYTOTOXIC T cells
CANCER cells
CANCER patients
Subjects
Details
- Language :
- English
- ISSN :
- 2076393X
- Volume :
- 9
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Vaccines
- Publication Type :
- Academic Journal
- Accession number :
- 150435309
- Full Text :
- https://doi.org/10.3390/vaccines9040334