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Identification of microbial markers across populations in early detection of colorectal cancer.

Authors :
Wu, Yuanqi
Jiao, Na
Zhu, Ruixin
Zhang, Yida
Wu, Dingfeng
Wang, An-Jun
Fang, Sa
Tao, Liwen
Li, Yichen
Cheng, Sijing
He, Xiaosheng
Lan, Ping
Tian, Chuan
Liu, Ning-Ning
Zhu, Lixin
Source :
Nature Communications; 5/24/2021, Vol. 12 Issue 1, p1-13, 13p
Publication Year :
2021

Abstract

Associations between gut microbiota and colorectal cancer (CRC) have been widely investigated. However, the replicable markers for early-stage adenoma diagnosis across multiple populations remain elusive. Here, we perform an integrated analysis on 1056 public fecal samples, to identify adenoma-associated microbial markers for early detection of CRC. After adjusting for potential confounders, Random Forest classifiers are constructed with 11 markers to discriminate adenoma from control (area under the ROC curve (AUC) = 0.80), and 26 markers to discriminate adenoma from CRC (AUC = 0.89), respectively. Moreover, we validate the classifiers in two independent cohorts achieving AUCs of 0.78 and 0.84, respectively. Functional analysis reveals that the altered microbiome is characterized with increased ADP-l-glycero-beta-d-manno-heptose biosynthesis in adenoma and elevated menaquinone-10 biosynthesis in CRC. These findings are validated in a newly-collected cohort of 43 samples using quantitative real-time PCR. This work proves the validity of adenoma-specific markers across multi-populations, which would contribute to the early diagnosis and treatment of CRC. The gut microbiome plays an important role in colorectal carcinogenesis and predictive microbiome signatures have been proposed for colorectal cancer (CRC) diagnosis. Here the authors perform a meta-analysis of 16S rRNA-based profiles to identify microbial markers able to discriminate patients with adenoma from control and CRC, building a model that can be applied for the early detection of CRC. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Complementary Index
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
150472259
Full Text :
https://doi.org/10.1038/s41467-021-23265-y