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Andrographolide Inhibition of Th17-Regulated Cytokines and JAK1/STAT3 Signaling in OVA-Stimulated Asthma in Mice.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 5/30/2021, p1-11, 11p, 1 Color Photograph, 2 Diagrams, 1 Chart, 5 Graphs
- Publication Year :
- 2021
-
Abstract
- Asthma has long been considered a disease of airway inflammation. The excessive or prolonged production of inflammatory mediators can result in airway remodeling and severe clinical syndromes such as dyspnea or even apnea. Therefore, pharmaceutical intervention is required to restrain the excessive release of such inflammatory mediators in control of asthma. Novel therapeutics and mechanistic insight are sought for the management of this chronic inflammatory disease. Andrographolide (AG) is a type of diterpenoid ester compound and is reported to demonstrate multiple properties such as antioxidation and anti-inflammation. However, the anti-inflammatory capacity of AG by regulating immunologic function in airway of asthma has not been fully studied to date. Therefore, this study investigates whether AG is capable of suppressing the inflammatory response of asthma in OVA-stimulated mice and the mechanism by which this is achieved. Animals were randomly divided into 4 groups: control group, OVA model group, OVA + AG (0.1 mg/ml) group, and OVA + dimethylsulfoxide (DMSO) group. The serum, BALF, and lung tissue of the mice were collected separately for the administration of ELISA, rt-PCR, western blot and pathological section and staining. We found that AG attenuated the OVA-induced production of IL-6, IL-17A, IL-17F, and RORĪ³t; inhibited the OVA-mediated phosphorylation of JAK 1 and STAT3; and alleviated airway remodeling and the neutrophil infiltration of lung tissue. We conclude that AG inhibits the inflammatory response of asthma in OVA-stimulated mice by blocking the activation of Th17-regulated cytokines and the JAK1/STAT3 signaling pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- DRUG therapy for asthma
ASTHMA prevention
CYTOKINES
INTERLEUKINS
TERPENES
ANIMAL experimentation
INFLAMMATION
ANTI-inflammatory agents
WESTERN immunoblotting
ANTIOXIDANTS
JANUS kinases
CELLULAR signal transduction
RATS
ENZYME-linked immunosorbent assay
DESCRIPTIVE statistics
TRANSCRIPTION factors
MOLECULAR structure
STATISTICAL sampling
POLYMERASE chain reaction
PHOSPHORYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 150573633
- Full Text :
- https://doi.org/10.1155/2021/6862073