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A new way to evaluate the germinative compartment in human epidermis, using [3H]thymidine incorporation and immunoperoxidase staining of 67 K polypeptide.
- Source :
- British Journal of Dermatology; Apr1983, Vol. 108 Issue 4, p433-439, 8p
- Publication Year :
- 1983
-
Abstract
- We have used tritiated thymidinc (<superscript>3</superscript>HT) labelling and immunoperoxidase staining of 67 K polypeptide in human epidermis to study normal skin before and after irritation induced by application of croton oil (20% and 50% in olive oil). By this double labelling method, it was possible to identify, on the same skin section, DNA-synthesizing nuclei and epidermal cells which contained 67 K polypeptide. Our results clearly indicate that the germinative compartment of normal epidermis is a mixed population comprising basal and adjacent suprabasal cells; as a rule, absence of expression of 67 K polypeptide, which characterizes all basal cells, could not be regarded as a good marker to distinguish the germinative from the differentiating compartments. In mild primary irritation, the ratio of <superscript>3</superscript>HT-labelled undifferentiated cells to keratinizing ones was similar to that observed in normal epidermis. In severe irritation, this homeostatic process was altered, as <superscript>3</superscript>HT-Iabelled cells were mainly 67 K negative. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00070963
- Volume :
- 108
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- British Journal of Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 15057417
- Full Text :
- https://doi.org/10.1111/j.1365-2133.1983.tb04595.x