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A comparative study on chitosan nanoparticle synthesis methodologies for application in aquaculture through toxicity studies.
- Source :
- IET Nanobiotechnology (Wiley-Blackwell); Jun2021, Vol. 15 Issue 4, p418-426, 9p
- Publication Year :
- 2021
-
Abstract
- Chitosan nanoparticles (CSNPs) have been recently used for various applications in aquaculture, especially as drug carriers. The aim of this study was to synthesise and investigate a superlative method of CSNP synthesis for application in aquaculture through aquaculture‐based toxicology screening methods. Two different methods were analysed: the first a direct ionic gelation method (A) and the other involving a low‐molecular‐weight chitosan microparticle intermediate method (B). Dynamic light scattering characterisation revealed that the CSNP particle sizes were 192.7 ± 11.8 and 22.9 nm from methods A and B, respectively. The LC50 values for brine shrimp toxicity were found to be 1.51 and 0.02 ppt in 24 h for methods A and B, respectively. Acute toxicity studies in Litopenaeus vannamei rendered LC50 values of 3235.94 and 2884.03 ppt in 24 h for methods A and B, respectively. Zebrafish toxicity studies revealed mortality rates of 21.67% and 55% at 20 mg/L concentration for methods A and B, respectively, with an increased expression of intracellular reactive oxygen species in method B. From these findings, it can be concluded that a comparatively reduced toxicity of CSNPs derived from ionic gelation method makes it more appropriate for application in aquaculture. [ABSTRACT FROM AUTHOR]
- Subjects :
- CHITOSAN
NANOPARTICLES
AQUACULTURE
DRUG carriers
DRUG delivery systems
Subjects
Details
- Language :
- English
- ISSN :
- 17518741
- Volume :
- 15
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- IET Nanobiotechnology (Wiley-Blackwell)
- Publication Type :
- Academic Journal
- Accession number :
- 150719256
- Full Text :
- https://doi.org/10.1049/nbt2.12047