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MiR-24-3p attenuates IL-1β-induced chondrocyte injury associated with osteoarthritis by targeting BCL2L12.
- Source :
- Journal of Orthopaedic Surgery & Research; 6/11/2021, Vol. 16 Issue 1, p1-10, 10p
- Publication Year :
- 2021
-
Abstract
- Background: MiR-24-3p has been reported to be involved in an osteoarthritis (OA)-resembling environment. However, the functional role and underlying mechanism of miR-24-3p in chondrocyte injury associated with OA remains unknown. Methods: The expression of miR-24-3p was determined using reverse transcription quantitative PCR analysis in OA cases and control patients, as well as IL-1β-stimulated chondrocyte cell line CHON-001. The cell viability was analyzed by CCK-8 assay. Apoptosis status was assessed by caspase-3 activity detection. The pro-inflammatory cytokines (TNF-α and IL-18) were determined using ELISA assay. The association between miR-24-3p and B cell leukemia 2-like 12 (BCL2L12) was confirmed by luciferase reporter assay. Results: We first observed that miR-24-3p expression level was lower in the OA cases than in the control patients and IL-1β decreased the expression of miR-24-3p in the chondrocyte CHON-001. Functionally, overexpression of miR-24-3p significantly attenuated IL-1β-induced chondrocyte injury, as reflected by increased cell viability, decreased caspase-3 activity, and pro-inflammatory cytokines (TNF-α and IL-18). Western blot analysis showed that overexpression of miR-24-3p weakened IL-1β-induced cartilage degradation, as reflected by reduction of MMP13 (Matrix Metalloproteinase-13) and ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs-5) protein expression, as well as markedly elevation of COL2A1 (collagen type II). Importantly, BCL2L12 was demonstrated to be a target of miR-24-3p. BCL2L12 knockdown imitated, while overexpression significantly abrogated the protective effects of miR-24-3p against IL-1β-induced chondrocyte injury. Conclusions: In conclusion, our work provides important insight into targeting miR-24-3p/BCL2L12 axis in OA therapy. [ABSTRACT FROM AUTHOR]
- Subjects :
- CARTILAGE cells
INTERLEUKINS
REVERSE transcriptase polymerase chain reaction
CYTOKINES
WESTERN immunoblotting
MICRORNA
APOPTOSIS
CELL survival
MATRIX metalloproteinases
OSTEOARTHRITIS
TUMOR necrosis factors
ENZYME-linked immunosorbent assay
GLYCOPROTEINS
MEMBRANE proteins
POLYMERASE chain reaction
CASPASES
Subjects
Details
- Language :
- English
- ISSN :
- 1749799X
- Volume :
- 16
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Orthopaedic Surgery & Research
- Publication Type :
- Academic Journal
- Accession number :
- 150853702
- Full Text :
- https://doi.org/10.1186/s13018-021-02378-6