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A compact fiber-integrated optofluidic platform for highly specific microRNA Förster resonance energy transfer detection.
- Source :
- Analyst; 7/21/2021, Vol. 146 Issue 14, p4454-4460, 7p
- Publication Year :
- 2021
-
Abstract
- MicroRNAs (miRNAs) have attracted extensive interest as promising biomarkers for the profiling of diseases. However, quantitative measurement of miRNAs presents a significant challenge in biochemical studies. In this work, we developed an innovative optofluidic platform to perform a rapid, simple, quantitative and high-specificity miRNA assay using the Förster resonance energy transfer (FRET) principle. A novel three-way junction FRET probe was proposed to enable rapid and enzyme-free miRNA detection. Using this platform, we performed one-step, amplification-free miRNA detection with simple device operation and achieved miRNA identification at a low concentration. The detection system could achieve high specificity for discrimination of three-base mismatches, and the sample volume was significantly reduced, favorable for low-level miRNA detection in material-limited samples. The establishment of a compact, low-cost, highly sensitive and selective miRNA analysis platform provides a valuable tool for point-of-care diagnosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- FLUORESCENCE resonance energy transfer
MICRORNA
Subjects
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 146
- Issue :
- 14
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 151363472
- Full Text :
- https://doi.org/10.1039/d1an00324k