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6-Shogaol from ginger shows anti-tumor effect in cervical carcinoma via PI3K/Akt/mTOR pathway.
- Source :
- European Journal of Nutrition; Aug2021, Vol. 60 Issue 5, p2781-2793, 13p, 1 Color Photograph, 6 Graphs
- Publication Year :
- 2021
-
Abstract
- Purpose: 6-Shogaol, an active phenolic compound from ginger (Zingiber officinale), can inhibit the growth of a variety of human cancer cells. Nevertheless, its underlying molecular mechanisms in cervical cancer remain unclear. In this study, we systematically examine the inhibitory effect of 6-shogaol on cervical cancer in vitro and in vivo. Methods: Cell proliferation was assessed by CCK8 assay and colony formation assay in HeLa and SiHa cells. We analyzed cell cycle and apoptosis through flow cytometry. GFP-LC3 puncta and transmission electron microscopy were used to observe autophagic bodies. Wound-healing assay and transwell assay were used for evaluating the migration of cells. Western blot was applied to detect protein expression levels. Results: 6-Shogaol could suppress cell proliferation and migration, cause cell cycle arrest in the G2/M phase in HeLa and SiHa cells. Moreover, 6-shogaol triggered the apoptosis process through the mitochondrial pathway by downregulating the expression levels of p-PI3K, p-Akt and p-mTOR. Further research indicated that the induction of apoptosis by 6-shogaol was remarkably decreased after the treatment of ROS scavenger and PI3K agonist. Additionally, 6-shogaol increased the number of LC3-positive puncta and autophagic bodies per cell in both HeLa and SiHa cells. Pretreatment of cells with Bafilomycin A1, an autophagy inhibitor, accelerated 6-shogaol mediated cell apoptosis, suggesting that induction of autophagy by 6-shogaol is suppressive to apoptosis. Furthermore, in vivo data revealed that 6-shogaol significantly inhibited tumor growth and cell proliferation in tumor tissues. Conclusion: These findings suggested that 6-shogaol could be developed as a functional food ingredient, which is potentially used as therapeutic agents for patients with cervical cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOLOGICAL models
IN vitro studies
FLOW cytometry
WOUND healing
GINGER
PHENOLS
IN vivo studies
PHOSPHOTRANSFERASES
ANIMAL experimentation
COLONY-forming units assay
AUTOPHAGY
WESTERN immunoblotting
ANTINEOPLASTIC agents
SIGNAL peptides
APOPTOSIS
RATS
CELL cycle
ELECTRON microscopy
GENE expression
CELL proliferation
CERVIX uteri tumors
PLANT extracts
MOLECULAR structure
BIOLOGICAL assay
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 60
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 151386821
- Full Text :
- https://doi.org/10.1007/s00394-020-02440-9