Ruania alkalisoli sp. nov., Isolated from Saline–Alkaline Soil.

A Gram-positive, strictly aerobic, ivory-colored, rod-shaped bacterial strain, designated RN3S43^T, was isolated from saline–alkaline soil, in Tumd Right Banner, Inner Mongolia, China. Strain RN3S43^T grew at 10–40 °C (optimum 30 °C), pH 6.0–10.0 (optimum pH 9.0), and 0–12.5% NaCl (optimum 2–4%). It was positive to oxidase, catalase, urease, and nitrate reductase. The methyl red and Voges–Proskauer tests were negative. The phylogenetic trees based on the 16S rRNA gene sequences and genome both showed that strain RN3S43^T clustered with Ruania alba YIM 93306 ^T and shared 95.5% and < 95.0% of 16S rRNA gene similarities with R. alba YIM 93306 ^T and all the other type strains. MK-8 was the major respiratory quinone. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unidentified lipid were the major polar lipids. The major fatty acids were anteiso-C_15:0 and iso-C_15:0. The genome consisted of a 4,448,708-bp circular chromosome, with a G+C content of 68.2%, predicting 3,911 coding sequence genes, 44 tRNA genes and two rRNA operons. The average nucleotide identity (ANI), amino acid identity (AAI), and digital DNA–DNA hybridization (dDDH) values of strain RN3S43^T to R. alba YIM 93306^T were 79.0%, 79.2%, and 23.4%, respectively. The results of phylogenetic, physiological, and phenotypic tests allowed the discrimination of strain RN3S43^T from its phylogenetic relatives. Ruania alkalisoli sp. nov. is therefore proposed, and the type strain is RN3S43^T (=CGMCC 1.18652^T = KCTC 49471^T). [ABSTRACT FROM AUTHOR]