Bulked segregant CGT‐Seq‐facilitated map‐based cloning of a powdery mildew resistance gene originating from wild emmer wheat (Triticum dicoccoides).

Bulked segregant CGT-Seq-facilitated map-based cloning of a powdery mildew resistance gene originating from wild emmer wheat (Triticum dicoccoides) In this study, we cloned the powdery mildew resistance allele I MlWE18 i ( I NLR SP WE18 sp i ) whose locus is not present in the wheat reference genomes using a combined BSA and CGT-seq strategies. Most of the cloned wheat disease resistance genes are NLRs that tend to be PAVs between the resistance and susceptible genotypes, especially the genes derived from wild relatives (Li I et al i ., 2020; Walkowiak I et al i ., 2020). Keywords: BSA; ChIP-Seq; NLR; PAV EN BSA ChIP-Seq NLR PAV 1288 1290 3 07/28/21 20210701 NES 210701 Powdery mildew, caused by I Blumeria graminis i f. sp. I tritici i ( I Bgt i ), is a widely occurring foliar disease of wheat worldwide. [Extracted from the article]