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Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.

Authors :
Kwon, Hyung Joon
Oh, Young Taek
Park, Saewhan
Kim, Sung Soo
Park, Jinju
Yin, Jinlong
Hong, Jun Hee
Kim, Chan Il
Ryu, Haseo
Park, Jong Bae
Lim, Min Kyung
Source :
PLoS ONE; 9/8/2021, Vol. 16 Issue 9, p1-12, 12p
Publication Year :
2021

Abstract

Introduction: Recently, electric cigarettes with liquid (e-liquid) were introduced as an alternative to tobacco smoking. They were promoted as possible cessation aids and were considered to be potentially less harmful than traditional tobacco-based cigarettes. However, there is little information on the toxicants present in e-liquids and their possible carcinogenic effects. Methods: Western blot analysis was performed to identify the protein levels of cancer progression related signal transducers. Patient-derived brain tumor cells (CSC2) were injected into mouse brains and tumor growth was then observed by performing magnetic resonance imaging (MRI) and hematoxylin and eosin (H&E) staining of the whole brain. Immunohistochemistry (IHC) staining and Immunofluorescence staining were performed to study the expression of pEGFR and pERK. Results: Western blotting revealed that e-liquids increased pEGFR and pERK expression in a dose dependent manner. Animal experiments revealed that the e-liquid treated group had accelerated tumor growth and poor prognosis compared to the vehicle group. Histological staining showed activation of pEGFR and pERK in the e-liquid treated group. Conclusion: Our study revealed that e-liquid activates pEGFR and pERK, leading to accelerated brain tumor growth and poor prognosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19326203
Volume :
16
Issue :
9
Database :
Complementary Index
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
152353191
Full Text :
https://doi.org/10.1371/journal.pone.0256730