Fluorescence-based PCR Detection of Goat Milk Powder Mixed with Cow Milk.

[Objectives] To amplify and identify the specific gene fragments of cattle and sheep by real-time fluorescence-based PCR, and quickly and accurately identify the adulteration problem of goat milk powder according to the differences of mitochondrial genes among different species. [Methods] The specificity, limit of detection and repeatability test were carried out by isolating somatic cells, extracting template DNA and determining the reaction conditions of real-time fluorescence-based PCR. [ Results] The real-time fluorescence-based PCR method could be used to identify the bovine and ovine-derived materials of milk powder with high specificity, high sensitivity and good repeatability, and the limit of detection of milk components in goat milk powder could reach 0.01%. [ Conclusions] The real-time fluorescence-based PCR method can be used to quickly and accurately detect the milk components in goat milk powder, and provide some technical support for the adulteration detection of dairy industry. [ABSTRACT FROM AUTHOR]